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Year : 2012  |  Volume : 26  |  Issue : 2  |  Page : 73-76

Prevalance of skin reactivity to fungal antigens in patients of nasobronchial allergy of Jalandhar and neighbouring area in Punjab

1 Consultant, Dermatologist and Allergist, Skin and VD Clinic and Allergy Centre, Jalandhar, Punjab, India
2 Department of Pharmacology, Punjab Institute of Medical Sciences, Jalandhar, Punjab, India
3 Department of Pharmacology, Civil Hospital, Jalandhar, Punjab, India

Date of Web Publication27-May-2013

Correspondence Address:
V P Jerath
Consultant Dermatologist and Allergist, Skin and VD Clinic and Allergy Centre, 180/4 Central Town, Jalandhar
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0972-6691.112551

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Atmospheric allergens play an important role in the pathogenesis of naso-bronchial allergy. It is important to know the prevalence of allergens in one's area.for proper diagnosis and effective management The data of positive reaction to fungi on 1500 patients of nasobronchial allergy has been included in this study. Allergy test (skin testing with intradermal injection of antigen) was conducted on patients and the results were interpreted by comparing the wheal produced by the test substance in relation to the control. While analyzing the results 60 patients were excluded because of (±) or one plus (+) cutaneous reaction to fungal antigens. Topping the list, cladosporium was positive in 158 patients (11%) followed by Alternaria tenuis in 144 (10%), Curvularia lunata in 108 (75%), Helminthosporium sp. in 115 (8%). Moderate positives were Rhizopus nigricans in 92 (6.4%) patients, Candida albicans in 86 (6%), Aspergillus fumigatus in 86 (6%), Mucor mucedo in 83 (5.8%), Aspergillus niger in 81 (5.6%), Phoma batae 79 patients (5.5%), Aspergillus tamarii 72 (5.5%), Trichoderma 72 (5%). The low positives were Penicillium in 50 patients(3.5%), Aspergillus flavus 43 (3%), Nigerospora 41 (2.5%), Neurospora sitophila 41 (2.5%), Epicoccum purpurascens 31 (22%), Aspergillus versicolor 30 (2%) and Fusarium solani 28 (2%) patients. Information about fungal antigen positivity is being provided in the present article, which would serve as a reference to the physicians dealing with allergy patients as well as those studying environmental sciences.

Keywords: Aerospora, fungal spores, fungi, nasobronchial

How to cite this article:
Jerath V P, Sood M, Nishchal R. Prevalance of skin reactivity to fungal antigens in patients of nasobronchial allergy of Jalandhar and neighbouring area in Punjab. Indian J Allergy Asthma Immunol 2012;26:73-6

How to cite this URL:
Jerath V P, Sood M, Nishchal R. Prevalance of skin reactivity to fungal antigens in patients of nasobronchial allergy of Jalandhar and neighbouring area in Punjab. Indian J Allergy Asthma Immunol [serial online] 2012 [cited 2023 Mar 26];26:73-6. Available from: https://www.ijaai.in/text.asp?2012/26/2/73/112551

  Introduction Top

Atmospheric allergens play a significant role in the pathogenesis of naso-bronchial allergy particularly rhinitis and asthma. Pollens, fungi, animal dander, house dust mite, and domestic pets are some of the important triggering factors for naso-bronchial allergy. Amongst the aeroallergens pollens have been studied maximally, followed by the fungi. The latter play an important role in disease causation.

Naso-bronchial allergy is prevalent amongst all the populations of the world. [1],[2],[3],[4] Epidemiological data suggests the prevalence of respiratory diseases from 15% to 30%. [5] In India 20% of the population has rhinitis and 15% develop bronchial asthma, according to a recent survey. [6] It is quite evident that the incidence has increased over the years, if we compare this with the earlier data. [7]

For a proper diagnosis and effective management it is important that one should be familiar with the prevalence of allergens in one's area. The purpose of the present study was to find out fungi detected by intradermal tests in Jalandhar and neighboring areas of Punjab state.

  Materials and Methods Top

Our study includes the data of positive reaction to fungi in 1500 patients aged 14-57 years of nasobronchial allergy. The data has been collected over a period of 29 years from 1982 to 2011. Out of 1500 patients (56% males and 44% females) 967 were suffering from nasal allergy, 500 from bronchial allergy and 33 patients from ocular allergy. The duration of illness varied from 1 year to 30 years. The allergy tests were conducted on patients who were not on antihistamines and steroids for 72 and 48 h respectively. Children lesser than 14 years, were not included because a good percentage of them may get automatic remission beyond this age.

Skin testing

Intradermal injection of antigen (1:500 dilutions) was administered on the arm or volar surface of the forearm. Each patient was tested with 0.01 ml of 1:500 concentration (w/v) extract of fungal antigen. A negative control with buffered saline and a positive control with 100 mcg/ml of histamine acid phosphate were used in the study. The skin reactions were read after 15-30 min and were graded according to the criteria already published. [8] The results were interpreted by wheal produced in relation to the negative control. Because of high incidence of one plus reaction in non-allergic individuals this group was excluded from the study, and only high positive were considered and analyzed.

  Results Top

Out of 1500 patients 1440 were taken for analysis. Sixty patients were excluded because of one plus or ± results.

[Table 1] depicts the results of cutaneous reactivity to various fungi (arranged in decreasing order of their allergenicity). Most of the fungi yielded 2 plus to 3 plus results. Cladosporium herbarum was positive in 158 (11%), Alternaria tenius in 144 (10%), Curvularia lunata in 108 (7.5%), Helminthosporium sp. 115 (8%), Rhizopus nigricans in 92 (6.4%), Candida albicans 86 (6%), Aspergillus fumigatus in 86 (6%), Mucor mucedo in 83 patients (5.8), and Aspergillus niger 81 patients (5.6%).
Table 1: Percentage positivity to various fungi in the descending order of positivity and number of patients (in brackets)

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Fungi showing less positive reaction were Penicillium sp., Neurospora sitophila, Nigrospora sp., Epicoccum purpurascens, Aspergillus versicolor, Fusarium solani and Aspergillus flavus.

  Discussion Top

There are a few studies on aerospora of Punjab. The "All India Co-ordinated project on Aerobiology" carried out in Amritsar [9] (1980-81) found C. herbarum, A. tenius, C. lunata, Helminthosporium sp., Nigrospora sp., Tetraploa, Aspergilli penicilli, F. solani and E. purpurascens as some of the major aero-spora. M. mucedo and Trichoderma were the minor ones. Phoma batae and R. nigricans were not detected. Our study is very much in consonance with the data obtained from "All India Project on Aerobiology."

Another aerial survey of fungal spores in Amritsar (Punjab state) by Sandhu and Randhawa demonstrated Aspergillus species to be the commonest fungus followed by Alternaria tenuis, C. lunata and F. solani.[10]

Another study on aerospora from Delhi by Rashmi Sharma et al. [11] on indoor and outdoor environments showed that species of Aspergillus, Cladosporium, Alternaria and Penicillium were the most commonly encountered fungi. The highest concentrations were recorded in January, April, and February in that order, while the lowest concentrations were observed in June when temperature in Delhi is high. The four major fungal spores found positive (I/D testing) in our study are C. herbarum (11%), A. tenuis (10%), Helminthosporium (8%), C. lunata (7.5%). The moderate positives are R. nigricans (6.4%), C. albicans (6%), A. fumigatus (5.9%), M. mucedo (5.8%), Asp niger (5.6%), P. batae 5.5%, Asp tamari 5%, Trichoderma 5%.

Another study (using cutaneous testing) from North India [12] had found positivity in the following order: C. lunata (9.2%), A. tenius (7.2%) P. batae (5.7%), A. fumigatus 5.6%, Nigrospora sitaphola 5.1%. Positivity of 2 to <5% was seen in the following order: M. mucedo 4.8%, Asp Niger 3.6%, Helminthosporium 3.6%, Trichoderma 2.4%. All these allergens played a significant role in causing naso-bronchial allergy. The study also observed that 3+ to 4+ reaction seen with one fungal antigen resulted in 2+ to 4+ reaction to 3-6 more fungal allergens. This shows an antigenic determinant being common to some fungi. This phenomenon was rarely observed in case of pollen antigens.

Rajendra Prasad et al. [6] in 2009 found high positivity to C. herbarum (10.2), A. tenuis 8.3, C. lunata 7.4% and Helminthosporium 11.1%. Their results varied from this study in positivity for A. fumigatus, A. flavus, M. mucedo, C. albicans, and F. solani [Table 2].
Table 2: Comparing the positivity of various fungi in different studies including the present one

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A study by Acharya [13] had similar positivity for C. herbarum, A. tenuis, C. lunata, and Helminthosporium [Table 2].

It may not be out of place to quote here that even in 1968 Agarwal et al. [14] on aeospora study found spores of A. tenuis, C. herbarum, C. lunata and Helminthosporium as the most frequent in that order.

Alternaria, Aspergillus, Cladosporium and F. Solani have been reported as important fungal allergens in India; [6],[14],[15] while recently Rashmi Sharma et al. [11] on skin prick tests found marked skin reactivity to Alternaria alternata (17.9%), followed by A. Fumigatus and Penicillium citrinum (15.5%) from Delhi region in children; while A. niger showed least marked skin reactivity. An almost similar positivity to fungal antigen was seen by Mangla Satputu [15] on aero spora from Shillong region. 3+ reactivity was observed in C. herbarum, A. tenuis, C. lunata, Helminthosporium, A. fumigatus, Asp tamari and 2+ and 1+ in M. mucedo and Trichoderma.

In Amritsar study by Singh et al. [9] Cladosporium herbarium is the most common in all areas of India followed by Alternaria (13 localities), C. lunata (12), Helminthosporium, and Nigerospora (10 localities).

The fungal spores like Phoma, M. mucedo, and R. nigricans have been observed in very few localities. All these studies show that the fungal spores do not vary much from region to region unlike the pollen grains. [16],[17]

  Conclusion Top

Thorough information about aerospora is already available and now information about fungal antigen positivity is being provided in the present article, which would serve as a reference to the physicians dealing with allergy patients as well as those studying environmental sciences.

  References Top

1.Pekkanen J, Remes ST, Husman T, Lindberg M, Kajosaari M, Koivikko A, et al. Prevalence of asthma symptoms in video and written questionnaires among children in four regions of Finland. Eur Respir J 1997;10:1787-94.  Back to cited text no. 1
2.Woolcock AJ, Bastiampillai SA, Marks GB, Keena VA. The burden of asthma in Australia. Med J Aust 2001;175:141-5.  Back to cited text no. 2
3.Anthracopoulos M, Karatza A, Liolios E, Triga M, Triantou K, Priftis K. Prevalence of asthma among schoolchildren in Patras, Greece: Three surveys over 20 years. Thorax 2001;56:569-71.  Back to cited text no. 3
4.Chhabra SK, Gupta CK, Chhabra P, Rajpal S. Prevalence of bronchial asthma in schoolchildren in Delhi. J Asthma 1998;35:291-6.  Back to cited text no. 4
5.Anonymous: All India Coordinated Project on Aeroallergens and Human Health. Report. New Delhi: Ministry of Environment and Forests; 2000.  Back to cited text no. 5
6.Prasad R, Verma SK, Dua R, Kant S, Kushwaha RA, Agarwal SP. A study of skin sensitivity to various allergens by skin prick test in patients of nasobronchial allergy. Lung India 2009;26:70-3.  Back to cited text no. 6
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7.Vishwanathan R. Definition, incidence, etiology and natural history of asthma. Indian J Chest Dis 1964;6:108-24.  Back to cited text no. 7
8.Shivpuri DN. Comparative evaluation of the sensitivity of common methods of diagnostic antigen tests in patients of respiratory allergy. Indian J Chest Dis 1962;3:102-7.  Back to cited text no. 8
9.Singh BP, Nair PK, Gangal SV, Joshi AP. Incidence of airborne pollen grains and fungi in different regions of India. Proc Nat Conf Environ Bio Pollution 1981;1:125-34.  Back to cited text no. 9
10.Sandhu DK, Randhawa IS. Studies on the air-borne fungal spores in Amritsar: Their role in keratomycosis. Mycopathologia 1979;68:47-52.  Back to cited text no. 10
11.Sharma R, Gaur SN, Singh VP, Singh AB. Association between indoor fungi in Delhi homes and sensitization in children with respiratory allergy. Med Mycol 2012;50:281-90.  Back to cited text no. 11
12.Shivpuri DN. Clinically important pollen, fungal and insect allergens for nasobronchial allergy patients in India. Asp Allergy Appl Immunol 1980;13:19-23.  Back to cited text no. 12
13.Acharya PJ. Skin test response to some inhalant allergens in patients of nasobronchial allergy from Andhra Pradesh. Asp Allergy Appl Immunol 1980;13:14-8.  Back to cited text no. 13
14.Agarwal MK, Shivpuri DN, Mukerji KG. Studies on the allergenic fungal spores of the Delhi, India, metropolitan area. Botanical aspects (aeromycology). J Allergy 1969;44:193-203.   Back to cited text no. 14
15.Satputu M, Dutta BK, Rao RR. Contributions to aerobiology of Shillong Studies on seasonal variation of atmospheric pollen and fungal spores. Proc Indian Natl Sci Acad 1983;49:675-86.  Back to cited text no. 15
16.Agashe SN. Public awareness of allergens. Indian J Allergy Asthma Immunol 2003;17:17-33.  Back to cited text no. 16
17.Singh AB, Kumar P. Common environmental allergens causing respiratory allergy in India. Indian J Pediatr 2003;17:17-33.  Back to cited text no. 17


  [Table 1], [Table 2]

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